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. 2006 May;141(1):135–146. doi: 10.1104/pp.106.076786

Figure 5.

Figure 5.

Distribution of mcSKD1 in microsomal fractions. A, Total cellular protein extracted from 10-d-old cultured ice plant cells were separated into soluble (S) and microsomal (M) fractions. Proteins were separated by SDS-PAGE (left) and immunoblotting against anti-mcSKD1 antiserum (right). Fifty micrograms of proteins were loaded per lane. The arrow indicates the position of a 60-kD immunoreactive protein. C, Total cellular protein; S, soluble protein; M, membrane protein. B, Microsomes isolated from cultured ice plant cells were fractioned using a continuous 8% to 45% Suc gradient, and nine fractions (numbers on the top) were collected from top to bottom. The average Suc concentration in each fraction is 11%, 14%, 18%, 20%, 23%, 28%, 34%, 39%, and 42% (w/v), respectively. Membrane proteins in each fraction were separated by SDS-PAGE and immunoblotting against anti-mcSKD1, mEH, anti-AtTLG2a, VSR, anti-BP80, and anti-V-ATPase antibody.