Figure 2.
HPLC analysis of carotenoids from E. coli TOP10F′ cells containing plant cDNAs and the Erwinia ΔcrtE construct. Besides pACCAR25ΔcrtE, double transformants contained either maize long clone pME14D1148 (A), maize short clone pME14D1268 (B), G. lutea pUCggpps (C), G. lutea pBS-FPS (D), or empty vector pBluescript II SK(−) (E). Absorption spectra and λmax for peaks 1 (zeaxanthin-β-d-diglucoside) and 2 (zeaxanthin) in section A are shown in panels F and G, respectively. Peaks 1 and 2 in all chromatograms displayed the same spectra.