Figure 5.

In vitro enzymatic activity of recombinant isoprenoid synthases from maize and H. brasiliensis. Cell-free extracts were prepared from E. coli cells containing plasmids encoding prenyl transferases and assayed by incorporation of [4-¹⁴C]IPP into short-chain isoprenoids, followed by dephosphorylation, reversed-phase TLC, and development of the chromatography plate in a Phosphorimager. Lane 1, Empty pBluescript II SK (−) vector; lane 2, pME14D1184 (maize long clone); lane 3, pME14D1268 (maize short clone); lane 4, pHBGG6 (H. brasiliensis GGPPS). Authentic isoprenol standards were run along with the enzymatic assay of isoprenol products and developed with p-anysaldehyde reagent.