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. 2006 May;141(1):220–231. doi: 10.1104/pp.106.077008

Figure 6.

Figure 6.

Effect of N terminus on GGPPS activity of maize isoprenoid synthases. Enzymatic assays were performed as described in “Materials and Methods” and Figure 5 legend. A, Cell extracts: lane 1, M15 cells with empty pQE30 vector; lane 2, TOP10F′ cells with pME14D1184; lanes 3 and 4, M15 cells with pQE1184UTR and pQE22ORF, respectively. B, Lane 5, His-tagged recombinant protein encoded by pQE1184UTR (the same construct used in A, lane 3), purified by affinity chromatography; lane 6, same as lane 1. C, Cartoon of constructs used in A and B.