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. 2006 May;141(1):196–207. doi: 10.1104/pp.106.079533

Table II.

Characterization of the identified vacuolar proteins

Accession numbers are from NCBI (http://www.ncbi.nlm.nih.gov/). Fractions are indicated by “x” in which each identified protein was present. The number of transmembrane domains (TMDs) is according to the prediction by SOSUI (http://sosui.proteome.bio.tuat.ac.jp/sosuiframe0.html). MW, Predicted Mr; % Cov., sequence coverage in percent; % Seq., sequence similarity of the closest Arabidopsis homologs; Ref., Arabidopsis homolog(s) found by Carter et al. (2004; a), Shimaoka et al. (2004; b), Szponarski et al. (2004; c), and Sazuka et al. (2004; d).

Accession No. Description Nontreated KIa NaOHb TMDs MW pI % Cov. ppc Arabidopsis Homologs % Seq. Ref.
Known vacuolar proteins
        gi|1051258 V-ATPase A-subunit x x x 0 64 5.3 77.8 1 At1g78900 90 a, b, c, d
        gi|167108 V-ATPase B-subunit (isoform 1) x x x 0 54 5.1 84.5 1 At1g76030 96 a, b, c
At4g38510 95 a, b
At1g20260 94 a, b
        gi|167110 V-ATPase B-subunit (isoform 2) x x x 0 53 5.1 81.6 1 At4g38510 97 a, b
At1g76030 96 a, b
At1g20260 95 a, b
        gi|12585487 V-ATPase C-subunit x x x 0 40 6.0 78.0 1 At1g12840 67 a, b, c
        gi|50929105 V-ATPase D-subunit x x x 0 29 9.8 33.1 1 At3g58730 76 a, b, c
        gi|4099148 V-ATPase E-subunit x x x 0 26 6.5 92.5 1 At4g11150 75 a, b, c
At1g64200 74 a, b
        gi|50916028 V-ATPase F-subunit x x x 0 14 5.6 16.9 1 At4g02620 84 a, b
        gi|50928483 V-ATPase G-subunit x 0 12 8.0 12.7 1 At3g01390 72 a, b
At4g23710 67 a
        gi|34898596 V-ATPase H-subunit x x x 1 51 7.1 11 1 At3g42050 71 a, b, c
        gi|18657017 V-ATPase subunit-a x x x 7 89 5.6 7.2 1 At2g21410 76 a, b, d
At4g39080 76 a, b, c, d
        gi|52353608 V-ATPAse subunit-c x x x 4 16 8.6 10.8 1 At1g19910 96 d
At2g16510 96 d
At4g34720 96 b, d
At4g38920 96 a
At1g75630 95 d
        gi|20160977 V-ATPAse subunit-d x x 0 40 4.8 27.6 1 At3g28715 90 a, b
At3g28710 89 a, b
        gi|11527561 Pyrophosphatase VP1 x x x 12 80 5.0 27.5 1 At1g15690 86 a, b, c, d
        gi|18274925 Pyrophosphatase AVP3 x x x 12 79 5.2 30.6 1 At1g15690 86 a, b, c, d
        gi|520936 γ-TIP-like protein x x x 6 25 6.0 10.4 1 At2g36830 74
At3g26520 74 a
At4g01470 68
        gi|12278525 Putative ABC transporter TAP2 x x x 5 69 8.5 9.3 1 At5g39040 77 a, b
        gi|22022396 ABC transporter TaMRP1 x 6 85 5.5 10.5 1 At3g62700 74 a, b
At2g47800 72 a, b, c
        gi|27263148 Putative ABC transporter MRP2 x x x 14 183 6.3 11.8 1 At2g34660 72 b
At1g30400 70 a, b, c, d
At1g30410 69
        gi|39545849 Two-pore calcium channel x x 12 85 5.4 10.4 1 At4g03560 59 a, c
        gi|28201131 Na+/H+ antiporter HvNHX1 x 9 59 7.6 8.2 0.98 At3g05030 75
At5g27150 73
        gi|50911128 Putative cytochrome b-561 x 6 27.6 6.6 9.1 0.97 At4g25570 60 a, b
Putative vacuolar transport proteins
        gi|7024413 Suc transporter HvSUT2 x x x 11 54 8.9 7.4 1 At1g09960 62
        gi|26986186 Hexose transporter HvSTP1 x 12 79 4.8 2.0 1 At4g35300 71 a
At1g20840 62 a
        gi|55773917 Putative ABC transporter x 10 149 6.0 1.9 0.93 At2g07680 59
        gi|34912536 Putative ABC transporter (PDR-like) x 14 162 8.0 1.5 1 At1g15520 68
At2g36380 60
At1g15210 58
        gi|34915038 Putative ABC transporter x x 9 162 10.7 1.7 1 At2g47800 50 a, b, c
At3g62700 49 a, b
        gi|34898286 Canalicular multispecific organic anion transporter 2-like protein x 15 164 7.8 1.7 0.91 At3g59140 65
        gi|15076661 Putative peptide transporter x x x 11 65 5.1 11.3 1 At2g02040 69 a, b
At2g02020 68
At3g54140 62
        gi|34015349 Putative chloride channel (CLC family) x 8 86 8.8 3.0 0.97 At5g33280 65
At5g49890 59
        gi|47497044 Amino acid-like transporter x 8 31 9.1 4.5 0.98 At2g41190 66
        gi|49388943 Putative hexose transporter x 11 80 5.2 2.1 1 At4g35300 72 a
        gi|51854311 Putative sugar transporter x x x 10 53 9.1 4.4 1 At1g75220 73
At1g19450 71 a
        gi|31433313 Putative sugar transporter x 12 79 4.9 2.2 0.96 At4g35300 72 a
        gi|14275750 Putative calcium translocating P-type ATPase x x x 10 63 4,9 8,3 1 At3g57330 68 a, b, c
At2g41560 66 a
At4g37640 64
Putative vacuolar membrane proteins
        gi|50726573 Putative protein kinase Xa21 x 2 124 5.9 3.3 0.93 At3g47110 41
At3g47570 41
At3g47090 39
        gi|8886326 Vacuolar targeting receptor bp-80 x 2 69 5.4 4.8 0.97 At3g52850 70
At2g14740 68 a
At2g14720 68
        gi|50943395 Putative syntaxin of plants 52 x 1 26 8.6 10.3 1 At1g79590 63 a
At1g16240 62 a
        gi|37651973 Blue copper-binding protein x x 2 17 5.5 13 0.99 At3g27200 40
At2g32300 37
At2g44790 36
        gi|34913062 Putative calcium sensor protein x 1 31 4.9 2.9 1 At4g33000 77
        gi|41529149 Putative acid phosphatases x x x 1 29 8.6 18.4 1 At4g29270 45
At4g29260 44 a
At4g25150 41
        gi|41019551 Putative Cys protease x 1 41 5.8 5.0 0.99 At4g39090 67
At2g21430 64 a
At4g16190 62
        gi|34904294 Putative bark storage protein x x 1 36 5.6 5.7 1 At4g24340 38
        gi|53792725 EREBP-4-like protein x x 1 23 5.3 12.5 1 At1g47740 62
At4g17486 53
At5g47310 54
        gi|544242 Endoplasmin homolog precursor x 1 92 4.8 6.7 1 At4g24190 77 a
        gi|7288255 RNase S-like protein precursor x x x 1 27 8.8 52 1 At1g14220 36 a
At2g02990 34
At1g26820 34
        gi|23345048 Hypersensitive-induced reaction protein 4 x 1 32 5.2 27.8 1 At5g51570 76 a, b
        gi|50252990 SPX domain-containing-like protein x x 10 78 6.0 2.4 0.99 At1g63010 68
At4g22990 68
At4g11810 67
        gi|50726593 Putative nodulin-like protein x x 10 58 8.3 9.2 1 At4g34950 75
At2g16660 65
        gi|51854288 Putative integral membrane protein x x x 10 54 8.6 2.0 0.99 At1g75220 71
At1g19450 70 a
        gi|51536186 Translocation related protein x 4 42 9.1 3.8 0.98 At3g20920 59
        gi|50948653 Type 1 membrane-like protein x x 3 12 4.8 8.2 1 At3g24160 41
        gi|50929895 Unknown protein x 2 113 9.3 1.8 0.91 At1g22610 58
At4g11610 52
        gi|55771327 Unknown protein x 5 152 6.0 1.7 1 At5g11700 66 a, b
        gi|50926173 Unknown protein x 2 47 5.2 5.4 1 At1g16010 78 a
At1g80900 74
        gi|50399936 Expressed protein x 1 36 6.0 4.5 1 At5g06300 31
At3g53450 31
Putative vacuolar membrane-associated proteins
    Stress response
        gi|53791853 Putative propyzamide-hypersensitive 1 x 0 97 5.77 4.1 0.91 At5g23720 59
        gi|5822249 Zymogen of a barley vacuolar aspartic proteinase x 0 51 5.2 5.4 1 At1g11910 72 a, b
At1g62290 68 a, b
At4g04460 64
        gi|23345042 Hypersensitive-induced reaction protein 1 x x 0 31 5.3 45.8 1 At5g62740 87 a, b
At1g69840 85 b
        gi|18650668 Temperature stress-induced lipocalin x 0 22 5.5 5.3 0.99 At5g58070 74 d
        gi|50912867 Putative elicitor-inducible protein EIG-J7 x 0 20 6.1 4.7 0.98 At4g39730 55 a
At2g22170 52 a
        gi|28555904 NBS-LRR disease-resistance protein homolog x 0 98 7.0 2.9 0.94 At5g43470 31
At1g50180 30
At5g48620 30
        gi|124037 Bowman-Birk-type trypsin inhibitor x 0 14 8.77 10.5 0.99 At5g43060 31 b
At1g32190 30
At1g74670 30
        gi|50911973 Ethylene-responsive-like protein x 0 20 5.9 6.2 0.98 At5g14680 73
At3g01520 72
    Membrane fusion and remodeling
        gi|32308080 α-SNAP x x 0 12 4.3 87.5 1 At3g56190 63 a, b
At3g56450 54
        gi|47834379 Cycloartenol synthase x x x 0 86 6.1 1.8 1 At2g07050 74 a, b
        gi|50912455 Putative ADP-ribosylation factor x x 0 20 8.6 12.5 1 At5g37680 90
At5g67560 80 a
        gi|50943481 Putative α-soluble NSF attachment protein x 0 32 5.0 20.4 1 At3g56190 63 a, b
        gi|52077324 Putative GTP-binding protein x 0 26 5.2 3.8 0.93 At1g01200 65
    Other
        gi|51035342 Putative carotenoid cleavage dioxygenase 1 x x 0 7 4.3 28.4 0.99 At3g63520 77 a
        gi|1167957 O-Methyltransferase x 0 30 6.4 6.6 0.99 At5g54160 44
At1g33030 39
At1g51990 38
        gi|50900950 Putative gag-pol precursor x 0 206 8.9 3.0 1
        gi|34901992 Putative sulfolipid synthase x 0 46 9.0 4.6 1 At5g01220 71
        gi|37718792 Calcineurin B protein x 0 26 4.7 12.9 1 At4g26570 88 a
At5g55990 88 a
        gi|34904770 Putative esterase x 0 38 6.0 2.5 0.99 At5g23530 47
At5g27320 42
At3g05120 39
        gi|11360993 Gly dehydrogenase x 0 111 6.3 1.66 0.99 At4g33010 81
At2g26080 80
        gi|21954110 RNase S-like protein x 0 28 6.5 21.2 1 At1g26820 39
At2g02990 38 a
At1g14220 38
        gi|7576892 Fatty acid α-oxidase x 0 71 7.7 5.2 0.96 At3g01420 62
At1g73680 58
        gi|51592190 Nucleotide pyrophosphatase/phosphodiesterase x 0 39.9 5.5 3.4 0.92 At4g24890 73
At5g50400 73
At1g13750 66
        gi|21742152 Unknown protein x 0 35 9.3 11.0 1 At4g13010 64 a
        gi|38345613 Unknown protein x 0 33 5.0 4.4 1 At4g20410 67
        gi|55771325 Unknown protein x 0 47 5.77 1.8 0.99 At5g12010 59
At4g29780 55
        gi|42408418 Hypothetical protein x 0 16 11.5 10.8 0.99
        gi|49389235 Hypothetical protein x 0 22 11.6 13.7 0.98
        gi|47847977 Hypothetical protein x 0 14 5.0 16.5 0.97
        gi|50928857 Unknown protein x 0 78 6.8 6.3 0.95 At3g11590 44
At3g20350 42
At1g50660 39
        gi|27497206 Unknown protein x 0 66 5.6 1.0 0.94 At2g20370 67
        gi|52353607 Unknown protein x 0 45 8.4 4.2 1
        gi|37533500 Hypothetical protein x 0 71 6.0 3.7 0.92
a

Tonoplast fraction washed with 0.3 m KI.

b

Tonoplast fraction washed with 0.5 m NaOH.

c

Protein-Prophet scores (pp) above 0.9 were accepted; at this cutoff, the rate of false positive protein identifications is less than 10%.