Table I.
Summary of apple ESTs
Library Code | Library Description | Minimum Sequence Length | Average Sequence Length | Maximum Sequence Length | Total No. ESTs | No. ESTs Assigned to TCa | No. Singletons | Percentage of Singletons per Library |
---|---|---|---|---|---|---|---|---|
AAAA | Royal Gala 59 DAFBb fruit, seeds removed | 50 | 425 | 767 | 7,018 | 5,495 | 1,523 | 21.7 |
AAFA | Royal Gala apple skin peel, tree-ripened fruit 150 DAFB | 47 | 382 | 821 | 6,184 | 5,389 | 795 | 12.9 |
AAFB | Royal Gala apple skin peel, tree-ripened fruit 150 DAFB | 50 | 331 | 682 | 1,556 | 1,058 | 498 | 32.0 |
AAGA | Pinkie expanding leaf, normalized | 51 | 342 | 702 | 530 | 418 | 112 | 21.1 |
AAKA | Pinkie expanding leaf, normalized | 51 | 365 | 687 | 516 | 389 | 127 | 24.6 |
AALA | Royal Gala 150 DAFB fruit cortex | 50 | 348 | 721 | 5,282 | 4,130 | 1,152 | 21.8 |
AALB | Royal Gala 150 DAFB fruit cortex | 51 | 341 | 739 | 1,275 | 933 | 342 | 26.8 |
AAMA | Royal Gala spur bud autumn | 51 | 374 | 668 | 988 | 679 | 309 | 31.3 |
AANA | Royal Gala partially senescing leaf | 50 | 318 | 729 | 1,888 | 1,453 | 435 | 23.0 |
AAOA | Royal Gala phloem | 50 | 371 | 734 | 4,519 | 3,059 | 1,460 | 32.3 |
AAPA | Royal Gala 24 DAFB fruit | 50 | 482 | 771 | 2,649 | 2,003 | 646 | 24.4 |
AARA | Royal Gala partially senescing leaf | 50 | 451 | 770 | 8,838 | 6,412 | 2,426 | 27.4 |
AASA | Royal Gala 10 DAFB fruit | 51 | 553 | 767 | 4,860 | 4,169 | 691 | 14.2 |
AASB | Royal Gala 10 DAFB fruit | 187 | 562 | 777 | 4,240 | 3,945 | 295 | 7.0 |
AASC | Royal Gala 10 DAFB fruit | 200 | 440 | 697 | 405 | 378 | 27 | 6.7 |
AAUA | Royal Gala 87 DAFB fruit cortex | 182 | 511 | 748 | 2,458 | 2,361 | 97 | 3.9 |
AAWA | Royal Gala 59 DAFB seeds | 101 | 520 | 766 | 5,472 | 4,769 | 703 | 12.8 |
AAXA | Royal Gala 126 DAFB fruit core | 195 | 514 | 766 | 5,092 | 4,655 | 437 | 8.6 |
AAYA | Royal Gala 126 DAFB fruit cortex | 118 | 521 | 742 | 4,481 | 3,946 | 535 | 11.9 |
AAZA | M9 xylem | 97 | 506 | 756 | 5,004 | 3,914 | 1,090 | 21.8 |
ABAA | Pacific Rose spur buds | 139 | 512 | 736 | 789 | 600 | 189 | 24.0 |
ABAB | Pacific Rose spur buds | 105 | 589 | 751 | 486 | 378 | 108 | 22.2 |
ABBA | Pacific Rose spur buds | 201 | 524 | 740 | 1,078 | 886 | 192 | 17.8 |
ABBB | Pacific Rose spur buds | 226 | 591 | 753 | 495 | 403 | 92 | 18.6 |
ABCA | Royal Gala fruit stored at 0.5°C for 24 h | 186 | 523 | 756 | 4,693 | 4,215 | 478 | 10.2 |
ABDA | Royal Gala fruit stored for 24 h under low oxygen/high CO2 | 165 | 552 | 741 | 4,808 | 4,616 | 192 | 4.0 |
ABEA | Royal Gala seedling leaves infected with V. inaequalisc | 170 | 564 | 775 | 4,798 | 3,967 | 831 | 17.3 |
ABEB | Royal Gala seedling leaves infected with V. inaequalisc | 198 | 521 | 766 | 562 | 395 | 167 | 29.7 |
ABKA | Royal Gala temperature stress leaves | 128 | 539 | 770 | 1,075 | 927 | 148 | 13.8 |
ABLC | Braeburn cell culture 3 d after subculture | 72 | 573 | 764 | 4,715 | 4,082 | 633 | 13.4 |
ABMA | M9 phloem | 108 | 557 | 781 | 4,900 | 4,383 | 517 | 10.6 |
ABNB | Braeburn cultured fruit cells, boron exposed | 209 | 585 | 754 | 944 | 777 | 167 | 17.7 |
ABPB | M9 root tips | 86 | 562 | 760 | 4,813 | 3,963 | 850 | 17.7 |
ABQA | Royal Gala flower | 199 | 504 | 749 | 926 | 831 | 95 | 10.3 |
AEAA | Aotea expanding leaf | 51 | 349 | 758 | 1,665 | 1,524 | 141 | 8.5 |
AELA | Royal Gala young expanding leaf | 50 | 318 | 712 | 5,457 | 4,388 | 1,069 | 19.6 |
AENA | Northern Spy expanding leaf | 52 | 428 | 759 | 1,222 | 918 | 304 | 24.9 |
AEPA | Pinkie expanding leaf | 50 | 370 | 828 | 4,786 | 3,891 | 895 | 18.7 |
AFBC | Royal Gala preopened floral bud | 50 | 350 | 877 | 4,959 | 3,933 | 1,026 | 20.7 |
AOFA | Royal Gala 24 DAFB fruit | 51 | 344 | 777 | 1,077 | 782 | 295 | 27.4 |
AVBB | Royal Gala young shoot | 50 | 296 | 737 | 5,572 | 4,511 | 1,061 | 19.0 |
AVBC | Royal Gala young shoot | 57 | 544 | 790 | 17,967 | 15,874 | 2,093 | 11.6 |
AYFB | Royal Gala 10 DAFB fruit | 54 | 304 | 693 | 645 | 410 | 235 | 36.4 |
Total for all libraries combined | 47 | 468 | 877 | 151,687 | 17,460d | 25,478 | ||
Total no. of NR sequences 43,938 |
Number of ESTs from each library that have been assigned to a TC sequence (contig).
Days after full bloom.
These libraries also will contain fungal sequences derived from the pathogen V. inaequalis (K. Plummer, W. Cui, and M. Templeton, unpublished data).
Total number of unique TC sequences in the entire database (i.e. this figure is not additive).