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. Author manuscript; available in PMC: 2006 May 11.
Published in final edited form as: Eur J Immunol. 2005 Nov;35(11):3187–3195. doi: 10.1002/eji.200526064

Figure 3.

Figure 3

Alteration of ζ chain phosphorylation pattern in Th1 cells with disrupted CD4 function. Polarized 5C.C7 Th1 cells were exposed to antigen-presenting cells pre-pulsed with 30 μM PCC88–104 peptide in the absence or the presence of the indicated amount of anti-CD4 mAb. After 5 min of cell contact, the extent of tyrosine phosphorylation involving the TCR-associated ζ chains was evaluated by immunoblotting after cell lysis and immunoprecipitation with anti-CD3ε antibody. Results are representative of two experiments.