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PAD activities toward BA and peptides H3-1, H3-2, and H4
| Peptide | PAD4 (wild type) | PAD4 (R374A) |
|---|---|---|
| BA | 100 | 5.7 |
| Peptide H3-1 | 21.1 | 0.4 |
| Peptide H3-2 | 19.6 | 0.6 |
| Peptide H4 | 46.0 | 1.0 |
PAD activity is shown relative to the wild-type enzyme (49.8 units/mg−1), which was defined as 100.
