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. 2006 Mar 24;103(14):5379–5384. doi: 10.1073/pnas.0601026103

Fig. 2.

Fig. 2.

ROS generation in HUVECs at different O2 concentrations. (A) Representative confocal images of HUVECs exposed to two different O2 concentrations. (B) Fluorescence of DHE oxidation products, as measured by flow cytometry, from intact cells incubated with DHE indicating intracellular superoxide production in the presence or absence of l-NMMA and antioxidants (50 μM MnTBAP plus 1 mM ascorbic acid) at 21%, 3%, and 0.5% O2. MFI, mean fluorescence intensity. ∗, Significantly different from control (C) 21% (P < 0.05); +, significantly different from control (C) 3% (P < 0.05).