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Control of AMPK activation at 3% O2. AMPK phosphorylation was assessed by Western blotting after different treatments. (A) HUVECs were incubated for 2 h in the absence (C) or presence of a SOD mimetic (50 μM MnTBAP). (B) ρ0 cells lacking functional mitochondria were incubated at 3% O2 for 2 h. Representative results from at least three independent experiments are presented. Values in brackets are the estimations from densitometry analysis. C, control; AMPK, loading control.
