Table 1.
Bioenergetic assessment in HUVECs
| [O2] | ATP nmol/106 cells |
||||
|---|---|---|---|---|---|
| Control | 2DG | Rotenone | Antimycin | 2DG plus rotenone | |
| 21% | 11.3 ± 1.1 | 5.7 ± 0.9** | 9.6 ± 1.0** | 7.3 ± 0.5** | 0.6 ± 0.1** |
| 3% | 9.3 ± 1.7 | 5.3 ± 1.0** | 7.2 ± 1.4** | 7.8 ± 1.5 | 0.8 ± 0.3** |
| 1.5% | 9.1 ± 0.6* | 4.2 ± 0.2** | 9.3 ± 1.1 | 7.2 ± 1.3** | 0.6 ± 0.3** |
| <0.5% | 8.3 ± 1.2* | 2.9 ± 0.4** | 8.5 ± 1.1 | 7.7 ± 0.7 | 0.4 ± 0.1** |
Effects of glycolytic and mitochondrial inhibitors on [ATP], at different O2 concentrations. Treatments were carried out for 2 h at the indicated O2 concentration before [ATP] was determined by chemiluminescence. For details of procedures, see Materials and Methods. Data represent the mean ± SEM of at least four independent experiments. 2DG, 20 mM; rotenone, 0.5 μM, antimycin, 0.5 μM.
*, Significantly different from control at 21% (P < 0.05);
**, significantly different from respective control (P < 0.05).