Fig. 1.

The N-terminal cytoplasmic domain of PC1 (NTM-PC1) colocalizes with mTOR and colocalizes and interacts with tuberin. (A–D) Expression of NTM-PC1 was induced with DOX for 16 h before fixation and immunostaining with antibodies against the indicated proteins. Noninduced NTM-PC1 cells served as controls (C and D, −DOX). Grp94 and GM130 are resident proteins of the endoplasmic reticulum and Golgi apparatus, respectively. (E) FLM-PC1 and CTM-PC1 cells were cultured in the absence or presence of DOX for 16 h, the PC1 fusion proteins were immunoprecipitated by using an antibody against CD16, and binding proteins were analyzed by Western blot with the indicated antibodies. The starting material (MDCK lysate) serves as a control. [Scale bars, 3 μm (A–D).]