Fig. 1.

Comparison of the Ca²⁺ channel complex partially purified from different tissues or through different methods. Sucrose gradient fractionation of Ca²⁺ channel complexes and subsequent immunoblotting for Ca²⁺ channel subunits demonstrate that the size of partially purified Ca²⁺ channel complexes differs depending on tissue and purification method. (A) Sucrose gradient fractionation of WGA-bound Ca²⁺ channel complex from rabbit brains. (B) Sucrose gradient fractionation of WGA-bound Ca²⁺ channel complex from rabbit skeletal muscles (SKM). (C) Sucrose gradient fractionation of heparin-bound Ca²⁺ channel complex from rabbit brains. The numbers at the top indicate the fraction of the sucrose gradient from top to bottom. Molecular mass standards (×10⁻³) are indicated on the left side of the panels. (D) Densitometry of Ca²⁺ channel subunits from Western blots of sucrose gradient fractions of WGA-bound Ca²⁺ channel complex. Fraction #, fraction number of sucrose gradient; Sucrose %, percentage of sucrose.