Table 1.
Summary of the amino acid substitutions identified between the putative eIF4AI/eIF4AIII homologues from the main lineages of eukaryotic evolution
| Position: TbEIFAI | Substitution: 4AI > 4AIII | Secondary structure | Domain | Overall position in predicted tertiary structure |
|---|---|---|---|---|
| 93 | K/N/V > R | Loop | N-terminal | Near α5/exposed |
| 139 | Q/R > E/G | α 5 | N-terminal | Exposed |
| 146 | Q/A > E/D | α 5 | N-terminal | Exposed |
| 153 | V > S/Aa | β4 | N-terminal | Buried |
| 197 | F > Y | α8 | N-terminal | Partially exposed |
| 213 | M > L | Loop | N-terminal | Interface/next to Motif III/partially buried |
| 220 | L/V/I > M | α9 | N-terminal | Buried |
| 226 | R/N > T | Loop | C-terminal | Exposed |
| 256 | L > Fa | α 10 | C-terminal | Partially buried |
| 264 | E > Da | α 10 | C-terminal | Exposed |
| 303 | S/D > Pa | Loop | C-terminal | Exposed |
| 328 | V/L > Wa | Loop | C-terminal | Interface/Motif V/Buried |
| 388 | H/N > A/Sa | Loop | C-terminal | Exposed |
aThese substitutions but not the others are present in the S. cerevisae nucleolar protein Fal1p [may be related to the eIF4AIII proteins (45)].