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A, migration of LNCaP and LNCaP-C4–2 cells toward recombinant SPARC at different concentrations (50, 100, and 200 ng/well). Integrins and VEGF regulate cell migration toward SPARC. B, cell migration to SPARC after treatment with or without anti-integrins αvβ3, anti-αvβ5, anti-α5β1, anti-α2β1 monoclonal antibody, and cRGDfV peptide. C, effect of neutralizing antibodies to VEGF, VEGFR-2, and PDGF on migration to SPARC.
