Fig. 2.

HAd5-specific IgA ELISA antibodies in mice inoculated with HAd5. 6-8 week-old BALB/c mice (5 animals/group) were immunized A) intranasally (i.n.) or B) intraperitoneally (i.p.) either with PBS or once (1x)/twice (2x) with 1 x 10⁸ p.f.u. of HAd5. There was no difference between NE-Z and E-Z groups at this stage. Serum samples were collected on 3 and 5 weeks post-inoculation and analyzed to determine levels of virus-specific IgA antibodies by ELISA. Each point represents the geometric mean value for five animals. NE-Z, nonencapsulated AdCA36lacZ; E-Z, AdCA36lacZ encapsulated into alginate microoparticles.