Table 2.
Experimental Design
| No. | Group | Route of | Mice Inoculated With | Blood Collection | Tissue Collection | |||
|---|---|---|---|---|---|---|---|---|
| Inoculation | Day 0 | Day 21 | Day 35 | Day 21 | Day 35 | on Daya | ||
| 1 | Naive/NE-Z/i.n. | i.n. | PBS | AdCA36LacZ | Yes | 22 | ||
| 2 | Naive/E-Z/i.p. | i.n. | PBS | AdCA36LacZ in microspheres | Yes | 24 | ||
| 3 | HAd5/NE-Z/i.n. | i.n. | HAd5 | AdCA36LacZ | yes | 22 | ||
| 4 | HAd5/E-Z/i.n. | i.n. | HAd5 | AdCA36LacZ in microspheres | yes | 24 | ||
| 5 | HAd5-HAd5/NE-Z/i.n. | i.n. | HAd5 | HAd5 | AdCA36LacZ | yes | yes | 36 |
| 6 | HAd5-HAd5/E-Z/i.n. | i.n. | HAd5 | HAd5 | AdCA36LacZ in microspheres | yes | yes | 38 |
| 7 | Naive/NE-Z/i.p. | i.p. | PBS | AdCA36LacZ | yes | 22 | ||
| 8 | Naive/E-Z/i.p. | i.p. | PBS | AdCA36LacZ in microspheres | yes | 24 | ||
| 9 | HAd5/NE-Z/i.p. | i.p. | HAd5 | AdCA36LacZ | yes | 22 | ||
| 10 | HAd5/E-Z/i.p. | i.p. | HAd5 | AdCA36LacZ in microspheres | yes | 24 | ||
| 11 | HAd5-HAd5/NE-Z/i.p. | i.p. | HAd5 | HAd5 | AdCA36LacZ | yes | yes | 36 |
| 12 | HAd5-HAd5/E-Z/i.p. | i.p. | HAd5 | HAd5 | AdCA36LacZ in microspheres | yes | yes | 38 |
a
The lungs and trachea were collected from i.n. inoculated mice, whereas, the liver, spleen, kidneys, peritoneal cells and mesenteric lymph nodes were collected from i.p. inoculated animals.