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. Author manuscript; available in PMC: 2006 Nov 11.
Published in final edited form as: Oncogene. 2006 May 11;25(20):2937–2942. doi: 10.1038/sj.onc.1209325

Fig 3. Mutant Stk11/Lkb1 displayed reduced kinase activity in activating its down stream target, AMPK, in RPMI 2650 cells.

Fig 3

RPMI 2650 cell transfected with empty vector, wild-type or mutant Stk11/Lkb1 were treated with or without 0.1 mM H2O2 for 20 min. Western blotting was performed as previously described. Total cell extracts were immunoblotted with antibodies for phosphor-Thr-172 AMPK (p-AMPK) (Cell Signaling, 1:1,000 dilution), total AMPK (Cell Signaling, 1:1,000 dilution) and Stk11 (Cell Signaling, 1:1,000 dilution).