FIGURE 1.

Steps of kinetic model development for ErbB family of receptors. (A) Schematic representation of the model development process. The central item is a detailed kinetic model of associations among HER receptors that is intended to convert inputs on receptor expression levels and ligand dosages X to receptor dimerization pattern Y. Setting up of the kinetic model is described in detail in text. In addition, the model is used to design experiments for parameter estimation to facilitate the transition to the next phase of model refinement. (B) Schematic of two-tiered modeling approach to relate HER expression levels to cell phenotype. The model for the HER receptor family is used to predict the receptor dimerization patterns in the cell. A phenomenological equation is then used to link model predictions to the cell phenotype estimation.