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Sphingosine- and ceramide-induced efflux of vesicular aqueous contents. LUVs were prepared, composed of SM/PE/Ch (2:1:1, mol ratio) containing entrapped ANTS/DPX. Sphingosine or ceramide in ethanol were added at time 0. (A) Time course of efflux. (▴) Ethanol (control); (▪) + 10 mol % ceramide; (•) + 10 mol % sphingosine; and (○) + 15 mol % sphingosine. (B) Dose-response curves. Data at time = 6000 s. (▴) Ceramide; (•) sphingosine (average values ± SD, n = 3).
