Figure 3.

Northern blot analysis of bfGnRHRs expression by using ³²P-labeled bfGnRHR-1, bfGnRHR-2, and bfGnRHR-3 cDNAs: p, pituitary; fb, forebrain; hb, hindbrain. Total RNA (10 μg) prepared from each brain region in different season (January, hibernation; June, breeding; and September, after-breeding) was subjected to Northern blot analysis under high-stringency conditions. The different transcripts and the sizes for each receptor are indicated (Left). GAPDH was used as an internal control indicating equal loading. Exposure times were 2.5 days for Northern blots of bfGnRHR-2 and bfGnRHR-3 and 12 h for Northern blots of bfGnRHR-1 and GAPDH.