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Southern blot analysis of bullfrog genomic DNA. Ten micrograms of high molecular weight genomic DNA were cut with EcoRI, XbaI, PstI, and BamHI. Southern blot analysis was performed under high-stringency conditions by using 32P-labeled bfGnRHR-1, bfGnRHR-2, and bfGnRHR-3 full-length cDNAs.
