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Influence of macromolecular crowding on the photocycle of PYP. Symbols represent transient absorption kinetics at 450 nm of a dilute solution of PYP in MES buffer at pH = 7. The red line shows transient absorption kinetics of PYP at 450 nm in solution with ∼10 mM BSA, whereas the blue line corresponds to the transient absorption kinetics of a solution of PYP containing PEG 2000 at 200 g/l.
