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. 2006 May 11;34(8):2294–2304. doi: 10.1093/nar/gkl183

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© The Author 2006. Published by Oxford University Press. All rights reserved

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Northern blotting for miR-21 in Hela cells after transfection of 2′-O-MOE-PS miR-21 ASO. miR-21 ASO or a control ASO were transfected into Hela cells and levels of miR-21 were measured 24 h later. A control experiment in which miR-21 ASO was spiked into Hela cell lysates showed that the ASO did not interfere with miR-21 detection (spike). Cells mock transfected with low-serum media only (Optimem) or with media containing the transfection reagent (Lipofectin) also showed no reduction in miR-21 levels.