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. Author manuscript; available in PMC: 2007 Jun 1.
Published in final edited form as: Int J Oncol. 2006 Jun;28(6):1353–1360.

FIGURE 1. RNA interference decreased uPAR and uPA levels in IOMM-Lee cells.

FIGURE 1

Total cell lysates and conditioned media collected from cells were transfected with mock, EV/SV, pUR, pU or pU2. 10 μg of protein were separated on 12% SDS-PAGE and immunoblotted with anti-uPAR antibody (A). 50 μg of conditioned media were separated on 12% SDS-PAGE and immunoblotted with anti-uPA antibody (A). β-actin antibody was simultaneously immunoprobed for loading control. Fibrin zymography was performed as described in Material and Methods with 1 μg of conditioned media to detect uPA activity (B).