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0.5×106 IOMM-Lee parental cells or cells transfected with EV/SV, pU, pUR or pU2 were injected intracranially into nude mice. After 4 weeks, the mice were sacrificed, the brains removed and fixed in formaldehyde, paraffin embedded and sectioned followed by H&E staining (A). The stained sections were viewed under microscope and the tumor area determined in serial sections to approximately determine the intracranial tumor volume (B).
