LC-MS analysis of metabolites in transgenic E.
californica cells with Coptis SMT.
(A) Total ion chromatogram of extracts of wild-type and
transgenic E. californica cells. (B)
Chromatogram of the columbamine ion (m/z 388) in
wild-type and transgenic E. californica cells.
(C) Chromatogram of the sanguinarine ion
(m/z 332) in wild-type and transgenic E.
californica cells. Dotted line, wild type; solid line,
transgenic plant. The peaks of columbamine (time, 12.7 min) and
sanguinarine (time 13.8 min) are indicated by arrows. *, Peaks
that increased in intensity in transgenic cells; closed triangle, peaks
that decreased in intensity in transgenic cells.