TABLE 3.
Summary of percentages of cells by the five methods of maternal IVB collection
| Cell type | % of cells (mean ± SD) by methoda
|
P valueb | |||||
|---|---|---|---|---|---|---|---|
| Peripheral blood | Prick | Perfusion | Incision | Biopsy | Tissue grinding | ||
| Monocytes | 7.1 (3.2) | 9.5 (5.3) | 8.1 (6.7) | 11.9 (5.8) | 7.3 (5.2) | 15.6 (8.3) | <0.0001 |
| Lymphocytes | 80.4 (9.5) | 73.9 (9.5) | 57.6 (20) | 53.8 (17) | 45.4 (16) | 52.5 (19) | <0.0001 |
| Total T cells | 69.1 (10.2) | 58.9 (12.2) | 45.8 (16.4) | 43.3 (15.0) | 38.0 (14.2) | 41.4 (15.1) | <0.0001*** |
| CD4 T cells | 36.1 (8.1) | 28.2 (8.9) | 19.0 (8.7) | 28.2 (11.3) | 18.1 (8.5) | 19.6 (7.6) | <0.0001*** |
| CD8 T cells | 29.3 (8.6) | 27.1 (10.0) | 20.0 (7.8) | 16.3 (7.8) | 15.0 (6.9) | 19.1 (7.8) | <0.0001 |
| Memory CD4 T cells | 29.4 (9.1) | 21.1 (10.2) | 17.6 (8.0) | 15.5 (10.7) | 16.9 (9.6) | 15.6 (8.3) | 0.002*** |
| Memory CD8 T cells | 16.3 (7.5) | 16.3 (8.8) | 15.4 (9.1) | 8.6 (7.9) | 8.6 (10.3) | 13.2 (6.8) | <0.0001 |
| B cells | 11.1 (5.5) | 15.1 (11.7) | 10.8 (10.8) | 9.6 (6.7) | 8.2 (7.7) | 11.0 (10.8) | 0.004 |
| NK cells | 11.9 (6.9) | 13.7 (8.3) | 15.3 (9.6) | 16.2 (7.8) | 16.6 (7.9) | 13.9 (7.1) | <0.0001 |
a
Peripheral blood was used only as a reference. Results for monocytes were obtained from CD45/CD14 staining. Lymphocyte numbers were obtained from the FCS-SSC plot. All other results were obtained from lymphocyte gating.
b
P values derived from (F test) comparison of all five methods of IVB collection. ***, P < 0.05 for comparisons (sign test) between peripheral and prick cell numbers (data not shown).