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Influence of modifiers of membrane rigidity on transcription. (A) Blot of RNA from undifferentiated sporangia (SP) and cleaving sporangia (CS) treated with 35 mM DMSO (D), 10 mM benzyl alcohol (B), or no chemical (−). Blots were hybridized with specific probes for PinifC1, PinifC2, PinifC3, or actA. (B) Same as panel A except that the RNA was from a transformant expressing M82(C3-60S)::GUS, and a probe for GUS (gus) was employed.
