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Subnuclear localization of TbNopp140 and TbNoLP. (A and C) Cells fixed in −20°C methanol and labeled with NUMAG and either anti-TbNopp140 or anti-TbNoLP antibodies. (B and D) Cells fixed in paraformaldehyde and labeled with NUMAG and either anti-TbNopp140 or anti-TbNoLP antibodies. Scale bars, 2 μm. (E) RNAi-mediated knockdown of TbNoLP affects nucleolar morphology and localization of TbNopp140. Scale bars, 1 μm.
