TABLE 2.

Virulence-related genes down regulated during heat shock^a

Oligonucleotide	NCBI accession no.b	Identification	Inhibition (fold)c
EH-IP0234.1	17.t00056	Gal/GalNAc lectin 35 subunit Lgl-1	7.4
EH-IP0234.2	17.t00056	Gal/GalNAc lectin 35 subunit Lgl-1	6.5
EH-IP0234.3	17.t00056	Gal/GalNAc lectin 35 subunit Lgl-1	1.9
EH-IP0816	56.t00038	Gal/GalNAc lectin 35 subunit Lgl-3	2.5
EH-IP0796.1	53.t00006	Gal/GalNAc lectin subunit Igl1	1.8
EH-IP0796.2	53.t00006	Gal/GalNAc lectin subunit Igl1	2.2
EH-IP0100	119.t00005	Gal/GalNAc lectin subunit Igl2	6.6
EH-IP0491.2	L14815d	Gal/GalNAc lectin subunit Hgl-3	2.4
EH-IP0715	442.t00004	Peroxiredoxin	7.6
EH-IP0160	136.t00007	Peroxiredoxin	18.6
EH-IP1002	963.t00001	Peroxiredoxin	16.7
EH-IP0928.2	79.t00025	Cysteine protease 1	7.9
EH-IP0928.1	79.t00025	Cysteine protease 1	9.1
EH-IP0409	242.t00001	Cysteine protease 1	7.5
EH-IP0343.2	206.t00005	Cysteine proteinase 2	7.1
EH-IP0343.1	206.t00005	Cysteine proteinase 2	7.1
EH-IP0343.3	206.t00005	Cysteine proteinase 2	7.5
EH-IP0343.6	206.t00005	Cysteine proteinase 2	6.3
EH-IP0343.4	206.t00005	Cysteine proteinase 2	6.1
EH-IP0343.5	206.t00005	Cysteine proteinase 2	6.9
EH-IP1066	X87214e	Cysteine protease 3	1.3
EH-IP0626.2	358.t00002	Cysteine protease 8	6.0
EH-IP0626.1	358.t00002	Cysteine protease 8	7.6
EH-IP1070	AY156071f	Cysteine protease 13	2.6
EH-IP0261	180.t00007	Cysteine protease 17	1.7
EH-IP0288.2	191.t00018	Cysteine proteinase	1.2
EH-IP0254.1	18.t00027	Amoebapore C	7.5
EH-IP0254.2	18.t00027	Amoebapore C	5.2
EH-IP0149.2	132.t00016	20-kDa antigen	1.7
EH-IP0149.1	132.t00016	20-kDa antigen	1.4
EH-IP0216	160.t00008	Surface antigen ariel1	17.4
EH-IP0614	349.t00010	Galactokinase	16.4

^aNotice that the surface molecule ARIEL (an asparagine-rich E. histolytica antigen of unknown function) (12) was down regulated 17-fold by heat shock, whereas amoebapore C was down regulated fivefold. Galactokinase (with a 16-fold down regulation) catalyzes phosphorylation of galactose during its catabolism. The enzyme has attracted significant research attention because of its important metabolic role, notably in humans, where defects in the enzyme can result in the disease state referred to as galactosemia (6). In addition, galactokinase-like molecules have been shown to act as sensors for the intracellular galactose concentration and, under suitable conditions, to function as transcriptional regulators (17).

^bhttp://www.ncbi.nlm.nih.gov/gquery/gquery.fcgi?term=ENTAMOEBA.

^cRate of inhibition of gene transcription after heat shock.

^dHgl-3 allele published in reference 15.

^eCP3 sequence published in reference 4.

^fCP13 sequence published in reference 4.