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Expression and purification of recombinant protein L7/L12-Omp16. The expression of recombinant fusion protein L7/L12-Omp16 was analyzed by SDS-PAGE and Western blot assays. Lane 1, molecular size protein markers; lane 2, lysate of E. coli BL21(DE3) transformed with the vector pET32a(+); lane 3, lysate of E. coli BL21(DE3) transformed with pET32a(+)-L7/L12-Omp16; lane 4, the purified fusion protein; lane 5, immunoblotting assay for purified protein (molecular mass indicated at right).
