FIG. 5.
Quantitative ELISPOT analysis of IFN-γ-producing lymphocytes upon in vitro stimulation with different antigens. Spleen cells (4 × 106 cells/ml) from mice inoculated with DNA vaccines, protein vaccines, live RB51 strain, or pcDNA3.1 were stimulated in vitro with rL7/L12-Omp16 or RPMI 1640 medium. (A) The typical ELISPOT assay results from the groups stimulated in vitro with rL7/L12-Omp16. (B) Statistics for data from each group. Each sample was assayed in quadruplicate wells. Data represent the mean spot number ±SD from each group of five mice. *, P < 0.01 compared with PBS immunization control group; ## versus #, P < 0.01.