TABLE 1.

PCR primers for DNA vacine construction

Gene and primers	Primer sequencea	Restriction enzyme
L7/L12
FL	5′-GAGATATCATGGCTGATCTCG-3′	EcoRV
RL-1	5′-GCCCTCGAGTTACTTGAGTTC-3′	XhoI
RL-2	5′-TAGGATCCCTTGAGTTCAAC-3′	BamHI
OMP16
FO	5′-TCGGATCCATGCGCCGTATCCAGTC-3′	BamHI
RO	5′-TATCTCGAGTTACCGTCCGGCCCCGTTG-3′	XhoI

^aRestriction sites are underlined, and initiation and termination codons are shown in boldface.