FIG. 6.

Dose-dependent response of HAP on the intestinal short-circuit current of rat ileum loaded in a Ussing chamber. HAP was added in the absence or presence of EDTA to the Ringer's solution in an Ussing chamber, and the change in Isc was recorded for the next 2 h from the time the sample was added. (a) Response of Isc to increasing doses of 35-kDa HAP. (b) Response of Isc to increasing doses of 45-kDa HAP. There is an initial increase, but the Isc falls as the dose reaches beyond 40 μg/ml. Values are the means ± standard deviations (error bars) for triplicate experiments. Proteins purified from E. coli DH5α were used as controls.