FIG. 1.

Expression of the hbhA gene by M. tuberculosis in axenic culture as determined by real-time RT-PCR. M. tuberculosis strain Erdman was grown in liquid medium with gentle shaking at 37°C. (A) Growth curve as determined by CFU counting at the indicated time points. (B) 16S rRNA-normalized levels of ftsZ and hsp70 transcripts at the different time points shown in panel A. (C and D) 16S rRNA (C)- and ftsZ (D)-normalized levels of hbhA determined during in vitro growth. Three samples per time point were analyzed, and RT-PCR was carried out four times on each sample. Error bars show standard deviations.