FIG. 4.

Analysis and comparison of sequences upstream of N. gonorrhoeae (Ng) and N. meningitidis (Nm) lst. (A) The 5′lst upstream regions of 28 N. gonorrhoeae strains and 17 N. meningitidis strains were PCR amplified using primer pair FusBam F1 and FusBam R1, and the amplicons were resolved in a 1.5% agarose gel. (B) Nucleotide sequence comparison of the 5′lst regions of N. meningitidis MC58 and N. gonorrhoeae F62: DNA fragments absent or duplicated are indicated by dashes within or above the sequence, respectively. Individual base differences are indicated by asterisks. The −10 and −35 sequences are in boldface and underlined. The putative Shine-Dalgarno (SD) sequence for lst, and the initiation codons (IC) for lst and icd (isocitrate dehydrogenase) are italicized and in boldface. The transcriptional start sites (tsp) downstream of the −10 sites are in boldface, italic, and indicated with small arrows.