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β-Gal activity of N. gonorrhoeae (Ng) F62 and N. meningitidis (Nm) MC58 5′lst reporter fusions. N. gonorrhoeae F62 and N. meningitidis MC58 ⊄3 5′lst::lacZ fusions were constructed with primers FusBam F1 and FusBam R1 (Table 1) as described in Materials and Methods. β-Gal activity of each fusion was determined in N. gonorrhoeae and N. meningitidis as chromosomal integrates (A) and in E. coli XL1-Blue as plasmid fusions (B).
