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. 2006 May;74(5):2876–2886. doi: 10.1128/IAI.74.5.2876-2886.2006

FIG. 2.

FIG. 2.

Comparison of levels of generation of O2− induced by alpha-toxin and OAG in rabbit neutrophils. (A) Rabbit neutrophils (1.0 × 106 cells/ml) were incubated with various concentrations of alpha-toxin and OAG for the indicated periods. The generation level of O2− was determined as described in Materials and Methods. Symbols: control, ⋄; 2.5 nM alpha-toxin, ▵; 25 nM alpha-toxin, •; 5 μM OAG, ▴; 125 μM OAG, ○. A typical result from one of five experiments is shown. (B) Rabbit neutrophils (1.0 × 106 cells/ml) were incubated with or without alpha-toxin or OAG at 37°C for 3 or 12 min, respectively. Intracellular DG and OAG levels were determined as described in Materials and Methods. Values represent means ± standard error for five to six experiments. (C) Rabbit neutrophils (1.0 × 106 cells/ml) were incubated with or without 2.5 nM alpha-toxin in the presence of 5.0 μM OAG at 37°C for the indicated periods. Symbols: control, ⋄; alpha-toxin plus OAG, •; alpha-toxin, ○; OAG, ▴. A typical result from one of five experiments is shown.