Skip to main content
. 2006 May;74(5):2876–2886. doi: 10.1128/IAI.74.5.2876-2886.2006

FIG. 7.

FIG. 7.

Effect of anti-TrkA antibody on alpha-toxin-induced generation of O2− and alpha-toxin binding to rabbit neutrophils. (A) Rabbit neutrophils (1.0 × 106 cells/ml) were incubated with various concentrations of TrkA antibody and 1.0 μg/ml alpha-toxin at 37°C for 15 min. Symbols: control, ⋄; alpha-toxin, •; alpha-toxin plus 0.1 μg/ml anti-TrkA, ○; alpha-toxin plus 0.5 μg/ml anti-TrkA, ▴; alpha-toxin plus 1.0 μg/ml anti-TrkA, ▵; alpha-toxin plus 1.0 μg/ml normal rabbit IgG, ×. A typical result from one of five experiments is shown. (B) Rabbit neutrophils (1.0 × 106 cells/ml) were incubated with 125I-labeled alpha-toxin and various concentrations of anti-TrkA antibody or normal rabbit IgG at 37°C for 60 s. The binding of 125I-labeled alpha-toxin to the neutrophils was measured as described in Materials and Methods. A typical result from one of five experiments is shown. (C) Rabbit neutrophils (10 × 106 cells/ml) were incubated with various concentrations of 125I-labeled alpha-toxin at 37°C for 10 min. The lysated cells were immunoprecipitated using anti-TrkA antibody or normal rabbit IgG. The 125I-labeled alpha-toxin was identified by SDS-PAGE and autoradiography, and TrkA was detected by Western blotting (WB) using anti-TrkA antibody. A typical result from one of five experiments is shown.