FIG. 8.
Effect of NGF on generation of O2− induced by alpha-toxin. (A) Rabbit neutrophils (1.0 × 106 cells/ml) were incubated with or without 0.1 μg/ml alpha-toxin and various concentrations of NGF at 37°C for 15 min. Symbols: control, ⋄; alpha-toxin, •; alpha-toxin plus 10 ng/ml NGF, ○; alpha-toxin plus 25 ng/ml NGF, ▴; alpha-toxin plus 50 ng/ml NGF, ▵; 50 ng/ml NGF, ×. A typical result from one of five experiments is shown. (B) Rabbit neutrophils (1.0 × 106 cells/ml) were incubated with or without 5 nM OAG and 50 ng/ml NGF at 37°C for 15 min. Symbols: control, ⋄; OAG, ▴; NGF, ○; OAG plus NGF, (•). A typical result from one of five experiments is shown. (C) Rabbit neutrophils (1.0 × 106 cells/ml) were incubated with or without 50 ng/ml NGF at 37°C for the indicated periods. The extracted phospho-PDK1, phospho-PKCθ, and PDK1 were subjected to SDS-PAGE and Western blotting using specific antibodies. A typical result from one of five experiments is shown.