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Phosphorylation of PDK1 and PKCθ induced by H148G in rabbit neutrophils. Rabbit neutrophils (1.0 × 106 cells/ml) were incubated with or without 1.0 μg/ml H148G at 37°C for the indicated periods. The extracted phospho-PDK1 and phospho-PKCθ were subjected to SDS-PAGE and Western blotting using specific antibodies. A typical result from one of five experiments is shown.
