TABLE 1.
Oligonucleotide primers used in this study
| Primera | Plasmid | Primer sequenceb | Locationc |
|---|---|---|---|
| 1364-1 | pRdx1364WT, pRdxA1364MUT | 5′-CACGGCTCTAGATGGCTTCTAAGATCGCTAG-3′ | 1 to 20 |
| 1364-2 | p1364 | 5′-CACGGCTCTAGATGGCTATCGCTTTAACCCCAC-3′ | −146 to −124 |
| 1364-3 | pRdx1364WT, pRdxA1364MUT | 5′-GTACCCGGGTTATCCTTGAAATTGAACGC-3′ | 1012 to 1032 |
| 1364REV | p1364 | 5′-TCTTCGCTTAAAAGCCATTCTTC-3′ | 1082 to 1105 |
| HP0165-1 | pRdxA165WT, pRdxA165MUT | 5′-CACGGCTCTAGATGCGTTTCTCTATCTTTTTTAAAGTTGTCG-3′ | 1 to 31 |
| HP0165-2 | pRdxA165WT, p165 | 5′-GTACCCGGGTTAACCCCACAATTGGCTTTTTCTGTTCC-3′ | 1297 to 1326 |
| HP0165-4 | pRdxA165MUT | 5′-GTACCCGGGTCACGCTTTTATCCCCTTGAGCGAAGG-3′ | 499 to 526 |
| 165FOR | p165 | 5′-GCGATGCGAATATCTTTAGGGTAG-3′ | −318 to −295 |
| 244-1 | p244 | 5′-CGACAAGCTCCTCAAAGAACAAC-3′ | −222 to −199 |
| 244-2 | p244 | 5′-GCACATTGAAAAAATCGGTGCC-3′ | 1439 to 1461 |
| 73-1 | pUreAcat | 5′-GAAGGGCAGTTGTGCTAAACACC-3′ | −858 to −835 |
| 73-2 | pUreAcat, pRdxA-1 | 5′-GGAAGTGTGAACCGATTTGAACC-3′ | 410 to 433 |
| PADureA-1 | pRdxA-1 | 5′-GTAGCTCAGTTGGTAGAGCATACCTTGACATGG-3′ | −336 to −303 |
| 954-1 | pRdxA-1 | 5′-GGGGCAAGGATAGGATACGTTC-3′ | −667 to −645 |
| 954-3 | pRdxA-1 | 5′-CGTAGGGAACTTTAGGATCTTCAAAAACCC-3′ | 842 to 872 |
| 1432-3 | p1432cat | 5′-TTTCTGCCCTTAACCTCGCTC-3′ | −475 to −454 |
| 1432-4 | p1432cat | 5′-CCGTGTTGTTCTTCATGGTGTG-3′ | 509 to 531 |
a
Primers were used for the construction of the plasmids indicated.
b
Restriction sites (XbaI [TCTAGA] and SmaI [CCCGGG]) are underlined.
c
Locations refer to positions relative to the first nucleotide of the initiation codon. The target gene (HP number) for each primer is indicated in the primer designation.