FIG. 6.

Characterization of purified rPap31 binding to HUVECs by ELISA. (A) Binding of B. henselae whole cells, OMPs, rPap31, and a fetuin control to HUVEC monolayers. (B) Dose-dependent binding of rPap31 to HUVECs. Shown is binding of increasing amounts of rPap31 (diamonds) or a fetuin control (triangles) to HUVEC monolayers. (C and D) Inhibition of B. henselae binding to HUVEC monolayers following pretreatment of bacteria with mouse anti-Pap31 antibodies (C) or a control mouse serum (D). Experiments were performed as described in Materials and Methods. For all experiments, absorbance values are means from representative experiments performed in triplicate. Error bars show the range of absorbance values.