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L. pneumophila rpoS is essential for the biogenesis of replicative phagosomes. Shown are representative transmission electron microscopy images of A. polyphaga cells 24 h after infection by the L. pneumophila parental strain AA100 (A), the rpoS mutant Lex100 (B), or the complemented strain Lex100/pBRO (C). The white arrow in panel B indicates a phagosome that contains a partially degraded rpoS mutant. M, mitochondria.
