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. 2006 May;74(5):2742–2750. doi: 10.1128/IAI.74.5.2742-2750.2006

TABLE 4.

Calculation of amount of each MAb needed to achieve 50% maximal binding to PNAG in an ELISA

MAb 50% Maximal binding (ng/ml)a 95% Confidence interval (ng/ml) r2 goodness of fit
F598 17.66 16.28-19.15 0.9997
F628 84.75 76.38-94.03 0.9994
F630 1051 913.4-1209 1.0
a

Determined by nonlinear regression of data for IgG1 MAb binding to PNAG (as shown in figure 1A) by use of a sigmoidal curve-fitting function for a variable slope and plotting the log10 concentration of the MAb versus percent maximal binding.