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P. aeruginosa DNA stimulates IL-8 secretion in primary human airway epithelial cells. A. CuFi1 cells (derived from a CF patient) were incubated for 24 h with medium alone, 20 ng/ml TNF-α, 25 μg/ml of DNA from P. aeruginosa PAO1, P. aeruginosa PA578I, or human cells (S9). B. Primary NHBE cells. Values represent percent relative to control. Insets, absolute IL-8 values, in nanograms per milliliter. *, P < 0.05; **, P < 0.01 (ANOVA).
