FIG. 6.

Upregulation of IL-8 secretion by P. aeruginosa DNA in airway epithelial cells depends on p38 and ERK MAPK signaling. IB3-1 (A and C) and S9 (B) cells were incubated with medium alone (open bars) or with 25 μg/ml P. aeruginosa PAO1 DNA (gray bars) for 24 h in the absence or presence of 10 μM SB203580 (p38 inhibitor), 20 μM PD98059 (Erk signaling inhibitor), or 5 μM SP600125 (JNK inhibitor). Cells were preincubated for 2 h before the stimulation with SB203580 and PD98059 and for 30 min with SP600125. **, P < 0.01 (ANOVA); lines indicate compared samples.