FIG. 2.

Median relative quantity of mRNA for cytokines IFN-γ, TNF-α, and IL-10 in nodular and ulcerated Buruli lesions determined by real-time PCR. Threshold cycles of amplification for each cytokine target were normalized to 18S rRNA and quantified relative to a control human RNA. The median relative quantity is indicated by a horizontal line. P values refer to comparisons between nodules and ulcers for each cytokine using the Mann- Whitney test. A P value of <0.05 was considered a significant difference. *, an IFN-γ result not available for one nodule and one ulcer.