TABLE 2.
Cytokine mRNA expression in nodular and ulcerative Buruli lesions determined by real-time PCR
| Target cytokine or chemokine | Median relative quantity of cytokine mRNA (range) |
P valuea | |
|---|---|---|---|
| Nodules (n = 16) | Ulcers (n = 28) | ||
| IFN-γb | 21.63 (0.31-1,380) | 33.82 (1.13-341.3) | 0.42 |
| TNF-α | 0.58 (0.05-11.7) | 0.96 (0.03-14.4) | 0.22 |
| IL-12p35 | 3.19 (0-35.3) | 3.49 (0-334.3) | 0.47 |
| IL-12p40 | 1.01 (0-109.9) | 3.24 (0-149.6) | 0.09 |
| IL-1β | 266.9 (9.3-37,120) | 430.5 (3.7-348,300) | 0.17 |
| IL-15 | 18.51(4.5-225.2) | 19.23 (3.3-318.5) | 0.81 |
| IL-8 | 78.55 (4.9-18,950) | 456.7 (17.5-77,400) | 0.004 |
| IL-10 | 0.07 (0.02-11.24) | 0.16 (0.01-4.45) | 0.43 |
a
P values refer to comparisons between 16 nodules and 28 ulcers for each cytokine using the Mann-Whitney test. A P value of <0.05 was considered a significant difference.
b
IFN-γ results for 1 nodule and 1 ulcer were not available.